Anti Lamin Antibody What Is It

This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells.

Anti lamin antibody what is it.

Performed under reducing conditions. Anti lamin a antibody ab26300 at 1 µg ml lane 1. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. It is the best among many laminin function blocking preps we have tested.

Lamins a and c are alternatively spliced versions of the lmna gene. Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin. Lamin a c antibodies lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. That is cell receptor binding is markedly hindered.

Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx. Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda. These proteins are found in many different cell types in three different forms a b and c.

Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. Fisher pa journal of cell science 108 pt 9. Analogies with vertebrate a type lamins.

Anti lamin antibody products lamin is a reported alias of the human protein lamin a c encoded by the gene lmna. However based on bioassays it appears that the mix of antibodies in this purified polyclonal anti serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited. Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin. Site mapping using a monoclonal antibody.

Species predicted to react based on 100 sequence homology. Recombinant human lamin a protein at 0 01 µg secondary all lanes. Weber k journal of cell science 108 pt 10. Expression of drosophila lamin c is developmentally regulated.

These proteins are found in many different cell types in three different forms a b and c. Anti lamin antibodies are available from several suppliers. Goat anti rabbit igg h l hrp preadsorbed at 1 5000 dilution developed using the ecl technique.